top of page

Xylene (100%) - Pure for analytical purposes

What is xylene pure?


 This is xylene containing over 98% of xylene isomers (o-, m-, p-). It is unacceptable to call xylene "analytically pure" if it contains ethylbenzene or other impurities in larger quantities.




 Xylene (98-99%) - Pure available here




 We offer 100% pure xylene, distilled in glass stills, preventing the ingress of heavy metals and other contaminants. Xylene prepared this way is the purest form of this product, ensuring tissue saturation without contamination artifacts and more distinct staining.




 Why PURE xylene?


 1. Clarity of preparations (clearing):


    
 
  •  Xylene is used to remove paraffin and prepare the preparation for mounting under a slide.
    • 
 
  •  If xylene contains impurities (e.g. metals, organic residues), it may cause tissue clouding or insufficient cleaning , which will result in less sharp images under the microscope.
    • 
 
  •  Glass distilled xylene minimizes this risk – the tissues are transparent and the preparations look "clean" and clear.
    • 





 2. Staining result (staining quality):


    
 
  •  Chemical contaminants may interfere with dyes (e.g. hematoxylin, eosin) – weakening their effect or changing their colors.
    • 
 
  •  This results in pale, patchy or abnormal staining .
    • 
 
  •  Glass-distilled xylene ensures that the dyes work as they should and that the colors are correct and stable.
    • 





 3. Attaching the cover glass (mounting):


    
 
  •  If xylene has impurities, chemical reactions may occur with the mounting medium (e.g. resin, Canada balsam).
    • 
 
  •  This may lead to air bubbles , dulling or peeling of the glass over time .
    • 
 
  •  "Glass distilled" minimizes such problems - the preparations are durable and aesthetic.
    • 





 4. Archival stability of preparations:


    
 
  •  In histopathological work, preparations must be stored for years .
    • 
 
  •  Contaminated xylene may cause staining degeneration or tissue destruction over time (e.g. browning, loss of detail).
    • 
 
  •  Preparations prepared with pure xylene are stable for decades .
    • 





 Ethylbenzene is classified by the IARC as a probable carcinogen , while pure xylene is not classified as a carcinogen. Avoiding ethylbenzene in xylene reduces the hazards of working with this reagent.
      bottom of page