Anti-PAX8 (clone QR016)
PAX8 (Clone QR016) – Optimal Marker for Tumors of Mesodermal and Endodermal Origin
NordicQC Run 64, 2022 Evaluation: ✅ Optimal staining for clone QR016!
PAX8 – Characteristics and Function
PAX8 belongs to the PAX family of transcription factors, which regulate organ development during embryogenesis.
✔ Structurally related to PAX5 and PAX2.
✔ Its expression is highly regulated during fetal development and is essential for organogenesis in:
- Kidneys
- Müllerian-derived organs (fallopian tubes, uterus, cervix)
- Thyroid
PAX8 Expression in Tumors
🔹 Transcription factors often diminish in mature tissues but can be re-expressed organ-specifically during malignant transformation.
🔹 Due to its restricted expression in normal tissues, PAX8 is a sensitive and specific marker for primary and metastatic tumors originating from:
- Thyroid – papillary carcinoma, follicular carcinoma
- Thymus
- Ovaries – serous and endometrioid carcinoma
- Endometrium – endometrial carcinoma
- Cervix (endocervical origin)
- Fallopian tubes
- Kidneys – renal cell carcinoma, papillary renal carcinoma
🔹 PAX8 is a nuclear marker, providing high sensitivity and specificity in histopathological diagnostics.
Summary
✔ PAX8 is a key marker for tumors originating from the thyroid, genitourinary system, and kidneys.
✔ Particularly useful in diagnosing both primary and metastatic tumors.
✔ Nuclear expression of PAX8 allows for easy detection in immunohistochemical (IHC) analysis.
Literature
[1] Chai H-J et al. (2017). Oncol Lett. 14(5): 5871-5.
[2] Ozcan A et al. (2011). Mod Pathol. 24(6):751-64.
[3] Bellevicine C et al. (2013). Eur J Endocrinol. 169(3):307-11.
[4] Tong GX et al. (2009). Mod Pathol. 22(9):1218-27.
[5] Plachov D et al. (1990). Development. 110(2):643-51.
[6] Poleev A et al. (1992). Development. 116(3):611-23.
[7] Wang Y et al. (2013). J Hematol Oncol. 6:60.
Advantage of QUARTETT Antibodies
ADVANTAGES OF RECOMBINANT RABBIT MONOCLONAL ANTIBODIES
Recombinant rabbit monoclonal antibodies—referred to as Q-clones—combine the best properties of both murine monoclonal and rabbit polyclonal antibodies, offering a broader diagnostic potential.
Key Advantages of Our Next-Generation Recombinant Rabbit Monoclonal Antibodies
✔ High Affinity due to rabbit origin, enabling greater sensitivity in assays—these antibodies bind strongly to antigens and maintain their bond even under challenging conditions, unlike low-affinity antibodies.
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✔ Expanded Antigen Recognition—better recognition of diverse antigens and epitopes.
✔ Target Epitopes Poorly Recognized by Mouse-Derived Antibodies, improving detection in certain applications.
✔ Enhanced Response to Small-Sized Epitopes, making them ideal for challenging targets.
✔ Significantly Improved Recognition of Murine Antigens, broadening their usability in research and diagnostics.
✔ Lower Background Staining, ensuring cleaner and more reliable results.
Recombinant vs. Hybridoma Antibody Generation
✔ Exceptional Consistency, Specificity, and Sensitivity—eliminating risks of gene loss, mutations, or cell line drift.
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Recombinant rabbit monoclonal antibodies represent the future of immunohistochemistry, offering unmatched reliability and precision in diagnostic and research settings.